9JLR

Crystal structure of GH57 family amylopullulanase from Aquifex aeolicus mutant E256Q in complex with maltopentaose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Glycoside hydrolase family 57 N-terminal domain-containing protein	polymer	485	58323.4	4	UniProt (O66934) Pfam (PF03065)	Aquifex aeolicus VF5
2	E, F, G, H (E, F, G, H)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		828.7	4	In PDB BIRD (PRD_900030) GlyTouCan (G50146AM)
3	I, J, K, L (A, B, C, D)	water	water		18.0	51	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 477 (UniProt: O66934)

PDB	External Database	Details
Gln 256	Glu 256	engineered mutation
Leu 478	-	expression tag
Glu 479	-	expression tag
His 480	-	expression tag
His 481	-	expression tag
His 482	-	expression tag
His 483	-	expression tag
His 484	-	expression tag
His 485	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	233293.7
Branched	Number of molecules	4
	Total formula weight	3314.9
All*	Total formula weight	236608.5

*Water molecules are not included.