9JHP

Cryo-EM structure of GPR4 complexed with miniG13 in pH6.8

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein subunit alpha-13	polymer	230	26574.4	1	UniProt (Q14344) Pfam (PF00503)	Homo sapiens (human)	G alpha-13,G-protein subunit alpha-13
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	346	38045.6	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (S)	scFv16	polymer	286	30668.2	1		Homo sapiens (human)
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (R)	G-protein coupled receptor 4	polymer	374	42688.3	1	UniProt (P46093) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 6C.l,GPR6C.l

Sequence modifications

A: 16 - 57 (UniProt: Q14344)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Thr 4	-	expression tag
Val 5	-	expression tag
Ser 6	-	expression tag
Ala 7	-	expression tag
Glu 8	-	expression tag
Asp 9	-	expression tag
Lys 10	-	expression tag
Ala 11	-	expression tag
Ala 12	-	expression tag
Ala 13	-	expression tag
Glu 14	-	expression tag
Arg 15	-	expression tag
Asp 42	Gly 57	engineered mutation
Asn 43	Glu 58	engineered mutation

A: 66 - 230 (UniProt: Q14344)

PDB	External Database	Details
Gly 58	-	linker
Gly 59	-	linker
Ser 60	-	linker
Gly 61	-	linker
Gly 62	-	linker
Ser 63	-	linker
Gly 64	-	linker
Gly 65	-	linker
Asp 111	Ser 248	engineered mutation
Asp 114	Glu 251	engineered mutation
-	Asp 253	deletion
-	Gln 254	deletion
-	Val 255	deletion
-	Leu 256	deletion
-	Met 257	deletion
-	Glu 258	deletion
-	Asp 259	deletion
-	Arg 260	deletion
-	Leu 261	deletion
-	Thr 262	deletion
Asp 124	Ile 271	engineered mutation
Ala 208	Ile 355	engineered mutation
Ile 211	Val 358	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Ile -5	-	expression tag
Gly -4	-	expression tag
Arg -3	-	expression tag
Ala -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Phe 1	-	expression tag

R: 1 - 354 (UniProt: P46093)

PDB	External Database	Details
Glu 355	-	expression tag
Phe 356	-	expression tag
Leu 357	-	expression tag
Glu 358	-	expression tag
Val 359	-	expression tag
Leu 360	-	expression tag
Phe 361	-	expression tag
Gln 362	-	expression tag
Gly 363	-	expression tag
Pro 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	145837.7
All*	Total formula weight	145837.7

*Water molecules are not included.