9JH5

Activation mechanism of CYSLTR2 by C16:0 ceramide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Cysteinyl leukotriene receptor 2	polymer	346	39678.2	1	UniProt (Q9NS75) Pfam (PF00001)	Homo sapiens (human)	CysLTR2,G-protein coupled receptor GPCR21,hGPCR21,G-protein coupled receptor HG57,HPN321
2	B (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	59	6504.4	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
3	C (S)	scFv16	polymer	250	26640.6	1		Mus musculus
4	D (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	361	41724.4	1		Homo sapiens
5	E (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39489.2	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
6	F (N)	Nb35	polymer	151	16228.4	1		Lama glama
7	G (R)	N-((E,2S,3R)-1,3-DIHYDROXYOCTADEC-4-EN-2-YL)PALMITAMIDE	non-polymer		537.9	1	Chemie (16C)
8	H, I (R)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	170265.2
Non-Polymers*	Number of molecules	3
	Total formula weight	1311.2
All*	Total formula weight	171576.4

*Water molecules are not included.