9JG1

Cryo-EM structure of Adriforant-bound Histamine receptor 4 H4R at inactive state

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Histamine H4 receptor,Soluble cytochrome b562	polymer	427	48922.7	1	UniProt (Q9H3N8) UniProt (P0ABE7) Pfam (PF00001)	Homo sapiens (human)	H4R,HH4R,AXOR35,G-protein coupled receptor 105,GPRv53,Pfi-013,SP9144,Cytochrome b-562
2	B (H)	anti-BRIL Fab Heavy chain	polymer	222	24371.1	1		Homo sapiens (human)
3	C (L)	anti-BRIL Fab Light chain	polymer	226	23977.8	1		Homo sapiens (human)
4	D (R)	Adriforant	non-polymer		262.4	1	Chemie (A1EBW)

Sequence modifications

R: 1 - 207 (UniProt: Q9H3N8)
R: 383 - 487 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 389	Met 29	conflict
Ile 484	His 124	conflict
Leu 488	-	linker
Glu 489	-	linker
Arg 490	-	linker
Ala 491	-	linker
Arg 492	-	linker
Ser 493	-	linker
Thr 494	-	linker
Leu 495	-	linker

R: 496 - 575 (UniProt: Q9H3N8)

PDB	External Database	Details
Ala 378	-	linker
Arg 379	-	linker
Arg 380	-	linker
Gln 381	-	linker
Leu 382	-	linker
Phe 576	-	expression tag
Leu 577	-	expression tag
Lys 578	-	expression tag
Ile 579	-	expression tag
Phe 580	-	expression tag
Cys 581	-	expression tag
Ile 582	-	expression tag
Lys 583	-	expression tag
Lys 584	-	expression tag
Gln 585	-	expression tag
Pro 586	-	expression tag
Leu 587	-	expression tag
Pro 588	-	expression tag
Ser 589	-	expression tag
Gln 590	-	expression tag
His 591	-	expression tag
Ser 592	-	expression tag
Arg 593	-	expression tag
Ser 594	-	expression tag
Val 595	-	expression tag
Ser 596	-	expression tag
Ser 597	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	97271.6
Non-Polymers*	Number of molecules	1
	Total formula weight	262.4
All*	Total formula weight	97534.0

*Water molecules are not included.