9JG0

Cryo-EM structure of neuropeptide FF receptor 2 in the ligand-free state with BRIL fusion, anti-BRIL Fab, and nanobody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Isoform 2 of Neuropeptide FF receptor 2,Soluble cytochrome b562	polymer	526	60471.2	1	UniProt (Q9Y5X5) UniProt (P0ABE7) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 74,G-protein coupled receptor HLWAR77,Neuropeptide G-protein coupled receptor,Cytochrome b-562
2	B (H)	Anti-BRIL fab heavy chain	polymer	229	24321.1	1		Homo sapiens
3	C (K)	Anti-fab nanobody	polymer	131	14461.8	1		synthetic construct
4	D (L)	Anti-BRIL fab light chain	polymer	215	23483.1	1		Homo sapiens

Sequence modifications

R: 9 - 245 (UniProt: Q9Y5X5)

PDB	External Database	Details
Asp 1	-	expression tag
Tyr 2	-	expression tag
Lys 3	-	expression tag
Asp 4	-	expression tag
Asp 5	-	expression tag
Asp 6	-	expression tag
Asp 7	-	expression tag
Ala 8	-	expression tag

R: 251 - 355 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 257	Met 29	conflict
Ile 352	His 124	conflict
Leu 356	-	linker
Glu 357	-	linker
Arg 358	-	linker
Ala 359	-	linker
Arg 360	-	linker
Ser 361	-	linker
Thr 362	-	linker
Leu 363	-	linker

R: 364 - 517 (UniProt: Q9Y5X5)

PDB	External Database	Details
Ala 246	-	linker
Arg 247	-	linker
Arg 248	-	linker
Gln 249	-	linker
Leu 250	-	linker
His 518	-	expression tag
Met 519	-	expression tag
Gly 520	-	expression tag
Leu 521	-	expression tag
Glu 522	-	expression tag
Val 523	-	expression tag
Leu 524	-	expression tag
Phe 525	-	expression tag
Gln 526	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	122737.1
All*	Total formula weight	122737.1

*Water molecules are not included.