9JFZ

Cryo-EM structure of intermediate state GPR4 complexed with miniGs/q in pH7.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	262	30595.5	1	UniProt (P63092)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	346	38045.6	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	157	17057.3	1		Lama glama
5	E (R)	G-protein coupled receptor 4	polymer	374	42688.3	1	UniProt (P46093)	Homo sapiens (human)	G-protein coupled receptor 6C.l,GPR6C.l

Sequence modifications

A: 5 - 195 (UniProt: P63092)

PDB	External Database	Details
Met -8	-	initiating methionine
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Glu -1	-	expression tag
Asn 0	-	expression tag
Leu 1	-	expression tag
Tyr 2	-	expression tag
Phe 3	-	expression tag
Gln 4	-	expression tag
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation

A: 204 - 384 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	engineered mutation
Asp 252	Ser 252	engineered mutation
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 362	Ile 372	engineered mutation
Ile 365	Val 375	engineered mutation
Lys 370	Arg 380	engineered mutation
Leu 374	Gln 384	engineered mutation
Gln 375	Arg 385	engineered mutation
Asn 377	His 387	engineered mutation
Glu 380	Gln 390	engineered mutation
Asn 382	Glu 392	engineered mutation
Val 384	Leu 394	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Ile -5	-	expression tag
Gly -4	-	expression tag
Arg -3	-	expression tag
Ala -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Phe 1	-	expression tag

R: 1 - 354 (UniProt: P46093)

PDB	External Database	Details
Glu 355	-	expression tag
Phe 356	-	expression tag
Leu 357	-	expression tag
Glu 358	-	expression tag
Val 359	-	expression tag
Leu 360	-	expression tag
Phe 361	-	expression tag
Gln 362	-	expression tag
Gly 363	-	expression tag
Pro 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	136247.9
All*	Total formula weight	136247.9

*Water molecules are not included.