9JFY
Cryo-EM structure of Neuropeptide FF receptor 2 in complex with hNPSF and Gi
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Isoform 2 of Neuropeptide FF receptor 2 | polymer | 429 | 49709.1 | 1 | UniProt (Q9Y5X5) Pfam (PF00001) | Homo sapiens (human) | G-protein coupled receptor 74,G-protein coupled receptor HLWAR77,Neuropeptide G-protein coupled receptor |
| 2 | B (L) | Neuropeptide SF | polymer | 12 | 1367.6 | 1 | UniProt (O15130) | Homo sapiens | NPSF |
| 3 | C (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40415.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 4 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37728.2 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 6 | F (S) | single-chain antibody Fv fragment (scFv16) | polymer | 259 | 27784.9 | 1 | Mus musculus |
Sequence modifications
R: 9 - 420 (UniProt: Q9Y5X5)
L: 1 - 11 (UniProt: O15130)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asp 1 | - | expression tag |
| Tyr 2 | - | expression tag |
| Lys 3 | - | expression tag |
| Asp 4 | - | expression tag |
| Asp 5 | - | expression tag |
| Asp 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Ala 8 | - | expression tag |
| His 421 | - | expression tag |
| Met 422 | - | expression tag |
| Gly 423 | - | expression tag |
| Leu 424 | - | expression tag |
| Glu 425 | - | expression tag |
| Val 426 | - | expression tag |
| Leu 427 | - | expression tag |
| Phe 428 | - | expression tag |
| Gln 429 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Nh2 12 | - | amidation |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 164865.9 | |
| All* | Total formula weight | 164865.9 |
