9JFW

Cryo-EM structure of GPR4 complexed with Gs in pH6.8

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	402	46680.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	346	38045.6	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	157	17057.3	1		Lama glama
5	E (R)	G-protein coupled receptor 4	polymer	374	42688.3	1	UniProt (P46093) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 6C.l,GPR6C.l

Sequence modifications

A: 2 - 394 (UniProt: P63092)

PDB	External Database	Details
Met -7	-	initiating methionine
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Ile -5	-	expression tag
Gly -4	-	expression tag
Arg -3	-	expression tag
Ala -2	-	expression tag
Arg -1	-	expression tag
Gly 0	-	expression tag
Phe 1	-	expression tag

R: 1 - 354 (UniProt: P46093)

PDB	External Database	Details
Glu 355	-	expression tag
Phe 356	-	expression tag
Leu 357	-	expression tag
Glu 358	-	expression tag
Val 359	-	expression tag
Leu 360	-	expression tag
Phe 361	-	expression tag
Gln 362	-	expression tag
Gly 363	-	expression tag
Pro 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	152332.7
All*	Total formula weight	152332.7

*Water molecules are not included.