9JFU

Cryo-EM structure of inactive GPR4 with NE52-QQ57

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (H)	Mbp-Anti-bril fab heavy chain	polymer	611	66624.8	1		Homo sapiens
2	B (K)	Anti-fab nanobody	polymer	123	13390.6	1		Homo sapiens
3	C (L)	Anti-bril fab light chain	polymer	214	23353.9	1		Homo sapiens
4	D (R)	G-protein coupled receptor 4,Soluble cytochrome b562	polymer	486	55236.5	1	UniProt (P46093) UniProt (P0ABE7) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 6C.l,GPR6C.l,Cytochrome b-562
5	E (R)	NE52-QQ57	non-polymer		416.5	1	Chemie (A1L1E)

Sequence modifications

R: 1 - 210 (UniProt: P46093)

PDB	External Database	Details
Asn 63	Asp 63	engineered mutation

R: 216 - 320 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 222	Met 29	engineered mutation
Ile 317	His 124	engineered mutation
Leu 321	-	linker
Glu 322	-	linker
Arg 323	-	linker
Ala 324	-	linker
Arg 325	-	linker
Ser 326	-	linker
Thr 327	-	linker
Leu 328	-	linker

R: 329 - 466 (UniProt: P46093)

PDB	External Database	Details
Ala 211	-	linker
Arg 212	-	linker
Arg 213	-	linker
Gln 214	-	linker
Leu 215	-	linker
Glu 467	-	expression tag
Phe 468	-	expression tag
Leu 469	-	expression tag
Glu 470	-	expression tag
Val 471	-	expression tag
Leu 472	-	expression tag
Phe 473	-	expression tag
Gln 474	-	expression tag
Gly 475	-	expression tag
Pro 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag
His 479	-	expression tag
His 480	-	expression tag
His 481	-	expression tag
His 482	-	expression tag
His 483	-	expression tag
His 484	-	expression tag
His 485	-	expression tag
His 486	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	158605.8
Non-Polymers*	Number of molecules	1
	Total formula weight	416.5
All*	Total formula weight	159022.4

*Water molecules are not included.