9JEC

Cryo-EM structure of canine TNF-alpha complexed with nanobody TNF30K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Tumor necrosis factor	polymer	173	19123.7	3	UniProt (P51742) Pfam (PF00229)	Canis lupus familiaris (dog)	Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2,TNF-a
2	D, E, F (D, E, F)	nanobody TNF30K	polymer	131	14463.4	3		Lama glama

Sequence modifications

A, B, C: 1 - 157 (UniProt: P51742)

PDB	External Database	Details
Met -9	-	initiating methionine
Lys -8	-	expression tag
Lys -7	-	expression tag
Leu -6	-	expression tag
Leu -5	-	expression tag
Ile -4	-	expression tag
Ala -3	-	expression tag
Ala -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
His 162	-	expression tag
His 163	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	100761.3
All*	Total formula weight	100761.3

*Water molecules are not included.