9JEA

cryo-EM structure of M1 muscarinic acetylcholine receptor in complex with atropine

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Muscarinic acetylcholine receptor M1,de novo design protein	polymer	466	51835.4	1	UniProt (P11229) Pfam (PF00001)	Homo sapiens (human)
2	B (A)	[(1R,5S)-8-methyl-8-azabicyclo[3.2.1]octan-3-yl] (2S)-3-oxidanyl-2-phenyl-propanoate	non-polymer		289.4	1	Chemie (A1EBT)

Sequence modifications

A: 25 - 213 (UniProt: P11229)

PDB	External Database	Details
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag
Met 1	-	expression tag
Asn 2	-	expression tag
Thr 3	-	expression tag
Ser 4	-	expression tag
Ala 5	-	expression tag
Pro 6	-	expression tag
Pro 7	-	expression tag
Ala 8	-	expression tag
Val 9	-	expression tag
Ser 10	-	expression tag
Pro 11	-	expression tag
Asn 12	-	expression tag
Ile 13	-	expression tag
Thr 14	-	expression tag
Val 15	-	expression tag
Leu 16	-	expression tag
Ala 17	-	expression tag
Pro 18	-	expression tag
Gly 19	-	expression tag
Lys 20	-	expression tag
Gly 21	-	expression tag
Pro 22	-	expression tag
Trp 23	-	expression tag
Gln 24	-	expression tag
Lys 55	Glu 55	conflict

A: 214 - 367 (PDB: 9JEA)
A: 368 - 428 (UniProt: P11229)

PDB	External Database	Details
Ile 422	-	insertion
Asn 423	-	insertion
Tyr 429	-	expression tag
Ala 430	-	expression tag
Leu 431	-	expression tag
Cys 432	-	expression tag
Asn 433	-	expression tag
Ile 434	-	expression tag
Asn 435	-	expression tag
Pro 436	-	expression tag
Met 437	-	expression tag
Cys 438	-	expression tag
Tyr 439	-	expression tag
Ala 440	-	expression tag
Leu 441	-	expression tag
Cys 442	-	expression tag
Asn 443	-	expression tag
Ile 444	-	expression tag
Asn 445	-	expression tag
Pro 446	-	expression tag
Met 447	-	expression tag
Cys 448	-	expression tag
Tyr 449	-	expression tag
Ala 450	-	expression tag
Leu 451	-	expression tag
Cys 452	-	expression tag
Asn 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag
His 457	-	expression tag
His 458	-	expression tag
His 459	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	51835.4
Non-Polymers*	Number of molecules	1
	Total formula weight	289.4
All*	Total formula weight	52124.8

*Water molecules are not included.