9JDN

Crystal structure of alanyl-tRNA synthetase L219M mutant in complex with ATP and L-alanine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Alanine--tRNA ligase	polymer	435	48721.8	2	UniProt (A0A172UEB3)	Methylomonas sp. DH-1	Alanyl-tRNA synthetase,AlaRS
2	C, G, H (A, B)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	3	Chemie (ATP)
3	D, I (A, B)	ALANINE	non-polymer		89.1	2	Chemie (ALA)
4	E, M (A, B)	CITRIC ACID	non-polymer		192.1	2	Chemie (CIT)
5	F, L (A, B)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
6	J, K, N (B)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
7	O, P (A, B)	water	water		18.0	183	Chemie (HOH)

Sequence modifications

A, B: 1 - 429 (UniProt: A0A172UEB3)

PDB	External Database	Details
Met 219	Leu 219	engineered mutation
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	97443.6
Non-Polymers*	Number of molecules	12
	Total formula weight	2604.5
All*	Total formula weight	100048.2

*Water molecules are not included.