9JCQ

Cryo-EM structure of the proton-sensing GPCR (GPR4)-Gs protein complex at pH 7.4

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha	polymer	361	41879.5	1		Homo sapiens
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	518	56515.3	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	128	13885.4	1		Lama glama
5	E (D)	G-protein coupled receptor 4	polymer	362	41026.7	1	UniProt (P46093) Pfam (PF00001)	Homo sapiens (human)	G-protein coupled receptor 6C.l,GPR6C.l
6	F, G (D)	2-[[(2~{R})-3-acetyloxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxyethyl-trimethyl-azanium	non-polymer		300.3	2	Chemie (A1L35)
7	H, I (D)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
8	J (D)	water	water		18.0	3	Chemie (HOH)

Sequence modifications

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Met 1	-	initiating methionine
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag

B: 346 - 518 (PDB: 9JCQ)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	161036.8
Non-Polymers*	Number of molecules	4
	Total formula weight	1373.8
All*	Total formula weight	162410.7

*Water molecules are not included.