9JCO
Cryo-EM structure of the proton-sensing GPCR (GPR4)-Gs protein complex at pH 6.5
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha | polymer | 361 | 41879.5 | 1 | Pfam (PF00503) UniProt (by SIFTS) (A0A5A9NRD5) | Homo sapiens | |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 518 | 56515.3 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7729.9 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 4 | D (N) | Nanobody 35 | polymer | 128 | 13885.4 | 1 | Lama glama | ||
| 5 | E (R) | G-protein coupled receptor 4 | polymer | 362 | 41026.7 | 1 | UniProt (P46093) Pfam (PF00001) | Homo sapiens (human) | G-protein coupled receptor 6C.l,GPR6C.l |
| 6 | F, G (R) | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
| 7 | H, I (R) | 2-[[(2~{R})-3-acetyloxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxyethyl-trimethyl-azanium | non-polymer | 300.3 | 2 | Chemie (A1L35) | |||
| 8 | J, K (A, R) | water | water | 18.0 | 5 | Chemie (HOH) |
Sequence modifications
B: 7 - 345 (UniProt: P62873)
B: 346 - 518 (PDB: 9JCO)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Gly 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Leu 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Gln 6 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 161036.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1373.8 | |
| All* | Total formula weight | 162410.7 |
