9JBF
Cryo-EM structure of the human LYCHOS Y57A mutant in complex with cholesteryl hemisuccinate in the contracted state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Lysosomal cholesterol signaling protein | polymer | 878 | 97941.7 | 2 | UniProt (Q7Z3F1) Pfam (PF03547) Pfam (PF00610) | Homo sapiens (human) | LYCHOS,G-protein coupled receptor PGR22 |
| 2 | C, D, N, O (A, B) | CHOLESTEROL HEMISUCCINATE | non-polymer | 486.7 | 4 | Chemie (Y01) | |||
| 3 | E, I, P, T (A, B) | 1,2-Distearoyl-sn-glycerophosphoethanolamine | non-polymer | 748.1 | 4 | Chemie (3PE) | |||
| 4 | F, Q (A, B) | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
| 5 | G, J, L, R (A, B) | 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 790.1 | 4 | Chemie (PC1) | |||
| 6 | H, K, M, S (A, B) | O-[(R)-{[(2R)-2,3-bis(octadecanoyloxy)propyl]oxy}(hydroxy)phosphoryl]-L-serine | non-polymer | 792.1 | 4 | Chemie (P5S) |
Sequence modifications
A, B: 1 - 870 (UniProt: Q7Z3F1)
| PDB | External Database | Details |
|---|---|---|
| Ala 57 | Tyr 57 | engineered mutation |
| Ser 871 | - | expression tag |
| Arg 872 | - | expression tag |
| Glu 873 | - | expression tag |
| Asn 874 | - | expression tag |
| Leu 875 | - | expression tag |
| Tyr 876 | - | expression tag |
| Phe 877 | - | expression tag |
| Gln 878 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 195883.5 | |
| Non-Polymers* | Number of molecules | 18 |
| Total formula weight | 12041.4 | |
| All* | Total formula weight | 207924.8 |
