9JA3

Herpes simplex virus type 1 polymerase machinery in complex with duplex DNA, acyclovir triphosphate and calcium ions

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase	polymer	1235	136683.8	1	UniProt (G8HBE4) Pfam (PF03104) Pfam (PF00136) Pfam (PF11590) UniProt (by SIFTS) (P04293)	Human herpesvirus 1 (strain 17) (HHV-1 Human herpes simplex virus 1)
2	B (B)	DNA polymerase processivity factor	polymer	513	54288.2	1	UniProt (P10226) Pfam (PF02282)	Human herpesvirus 1 (strain 17) (HHV-1 Human herpes simplex virus 1)	DNA-binding protein UL42,Polymerase accessory protein,PAP
3	C (T)	DNA (27-MER)	polymer	27	8421.4	1		synthetic construct
4	D (P)	DNA (5'-D(*AP*GP*GP*CP*CP*AP*TP*AP*CP*GP*AP*TP*CP*CP*TP*TP*CP*CP*CP*CP*TP*AP*C)-3')	polymer	23	6921.5	1		synthetic construct
5	E, F, G (A)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
6	H (T)	ACYCLOVIR TRIPHOSPHATE	non-polymer		465.1	1	Chemie (AVP)

Sequence modifications

B: 1 - 488 (UniProt: P10226)

PDB	External Database	Details
Met -24	-	initiating methionine
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Asp 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	206315.0
Non-Polymers*	Number of molecules	4
	Total formula weight	585.4
All*	Total formula weight	206900.4

*Water molecules are not included.