9J9Z
Cryo-EM structure of Outward state Anhydromuropeptide permease (AmpG) complex with GlcNAc-1,6-anhMurNAc
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (H) | anti-BRIL Fab Heavy chain | polymer | 225 | 23904.7 | 1 | Homo sapiens | ||
| 2 | B (K) | anti-BRIL Fab Nanobody | polymer | 120 | 13159.4 | 1 | synthetic construct | ||
| 3 | C (L) | anti-BRIL Fab Light chain | polymer | 212 | 23209.8 | 1 | Homo sapiens | ||
| 4 | D (A) | Muropeptide transporter,Soluble cytochrome b562 | polymer | 589 | 64721.0 | 1 | UniProt (A0AB38FS76) UniProt (P0ABE7) Pfam (PF07361) | Yokenella regensburgei | Cytochrome b-562 |
| 5 | E (A) | (2R)-2-[[(1R,2S,3R,4R,5R)-4-acetamido-2-[(2S,3R,4R,5S,6R)-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]oxy-6,8-dioxabicyclo[3.2.1]octan-3-yl]oxy]propanoic acid | non-polymer | 478.4 | 1 | Chemie (2YP) |
Sequence modifications
A: 5 - 198 (UniProt: A0AB38FS76)
A: 203 - 303 (UniProt: P0ABE7)
A: 306 - 593 (UniProt: A0AB38FS76)
| PDB | External Database | Details |
|---|---|---|
| Trp 50 | Gly 50 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Trp 205 | Met 29 | engineered mutation |
| Ile 300 | His 124 | engineered mutation |
| Leu 304 | - | linker |
| Gly 305 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ala 199 | - | linker |
| Asp 200 | - | linker |
| Leu 201 | - | linker |
| Ala 202 | - | linker |
| Trp 372 | Leu 269 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 124994.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 478.4 | |
| All* | Total formula weight | 125473.3 |
