9J8O

Cryo-EM structure of BAF-Lamin A/C IgF-H1-nucleosome complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, O, S (A, E, a, e)	Histone H3.1	polymer	139	15719.4	4	UniProt (P68431) Pfam (PF00125)	Homo sapiens (human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
2	B, F, P, T (B, F, b, f)	Histone H4	polymer	106	11676.7	4	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
3	C, G, Q, U (C, G, c, g)	Histone H2A type 1-B/E	polymer	133	14447.8	4	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H, R, V (D, H, d, h)	Histone H2B type 1-J	polymer	129	14217.5	4	UniProt (P06899) Pfam (PF00125)	Homo sapiens (human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I, W (I, i)	DNA (193-MER)	polymer	193	59351.8	2		synthetic construct
6	J, X (J, j)	DNA (193-MER)	polymer	193	59823.1	2		synthetic construct
7	K, L, Y, Z (K, L, k, l)	Barrier-to-autointegration factor	polymer	93	10487.1	4	UniProt (O75531) Pfam (PF02961)	Homo sapiens (human)	Breakpoint cluster region protein 1
8	AA, M (M, m)	Lamin-A/C	polymer	158	17551.4	2	UniProt (P02545) Pfam (PF00932)	Homo sapiens (human)	70 kDa lamin,Renal carcinoma antigen NY-REN-32
9	BA, N (N, n)	Histone H1.1	polymer	221	22633.2	2	UniProt (Q02539) Pfam (PF00538)	Homo sapiens (human)	Histone H1a

Sequence modifications

A, E, a, e: 0 - 135 (UniProt: P68431)

B, F, b, f: 0 - 102 (UniProt: P62805)

C, G, c, g: 0 - 129 (UniProt: P04908)

D, H, d, h: 0 - 125 (UniProt: P06899)

K, L, k, l: 1 - 89 (UniProt: O75531)

M, m: 411 - 566 (UniProt: P02545)

PDB	External Database	Details
Ser 409	-	expression tag
His 410	-	expression tag

N, n: 1 - 215 (UniProt: Q02539)

Sequence viewer

Contents of the asymmetric unit

*Water molecules are not included.