9J63

Cryo-EM structure of neddylated Cul2-Rbx1-EloBC-FEM1B homodimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, D)	Cullin-2	polymer	751	87927.8	2	UniProt (Q13617) Pfam (PF00888) Pfam (PF10557)	Homo sapiens (human)	CUL-2
2	B, F (C, F)	Elongin-C	polymer	98	11045.7	2	UniProt (Q15369) Pfam (PF03931)	Homo sapiens (human)	EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1
3	C, G (B, G)	Elongin-B	polymer	104	11748.4	2	UniProt (Q15370) Pfam (PF00240)	Homo sapiens (human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
4	D, H (E, H)	E3 ubiquitin-protein ligase RBX1	polymer	108	12290.0	2	UniProt (P62877) Pfam (PF12678)	Homo sapiens (human)	E3 ubiquitin-protein transferase RBX1,Protein ZYP,RING finger protein 75,RING-box protein 1,Rbx1,Regulator of cullins 1,ROC1
5	I, J, K, L, M... (E, H)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

A, D: 1 - 745 (UniProt: Q13617)

PDB	External Database	Details
His 746	-	expression tag
His 747	-	expression tag
His 748	-	expression tag
His 749	-	expression tag
His 750	-	expression tag
His 751	-	expression tag

C, F: 16 - 112 (UniProt: Q15369)

PDB	External Database	Details
Met 15	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	246023.8
Non-Polymers*	Number of molecules	6
	Total formula weight	392.5
All*	Total formula weight	246416.2

*Water molecules are not included.