9J5O

Cryo-EM structure of TrhO from B. subtilis complexed with tRNA Ala

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	tRNA uridine(34) hydroxylase	polymer	352	40520.5	1	UniProt (O31457) Pfam (PF17773) Pfam (PF00581) Pfam (PF12368)	Bacillus subtilis subsp. subtilis str. 168	tRNA hydroxylation protein O
2	B (B)	RNA (76-MER)	polymer	76	24507.5	1		Bacillus subtilis subsp. subtilis str. 168
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 1 - 322 (UniProt: O31457)

PDB	External Database	Details
Met -29	-	initiating methionine
Ala -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
Gly -19	-	expression tag
Met -18	-	expression tag
Ala -17	-	expression tag
Ser -16	-	expression tag
Met -15	-	expression tag
Thr -14	-	expression tag
Gly -13	-	expression tag
Gly -12	-	expression tag
Gln -11	-	expression tag
Gln -10	-	expression tag
Met -9	-	expression tag
Gly -8	-	expression tag
Arg -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	65028.1
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	65093.5

*Water molecules are not included.