9J40

Cryo-EM structure of lysosome cholesterol sencing protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	G protein-coupled receptor 155,Lysosomal cholesterol signaling protein fusion protein	polymer	870	96994.7	2	UniProt (G1R1S1) UniProt (Q7Z3F1) Pfam (PF03547) Pfam (PF00610)	Nomascus leucogenys	LYCHOS,G-protein coupled receptor PGR22
2	C, F, G, J, M... (B, A)	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	6	Chemie (POV)
3	D, K (B, A)	3-SULFOOXY-1H-INDOLE	non-polymer		213.2	2	Chemie (IOS)
4	E, L (B, A)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
5	H, O (B, A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
6	I, P (B, A)	TRYPTOPHAN	non-polymer		204.2	2	Chemie (TRP)

Sequence modifications

B, A: 13 - 520 (UniProt: G1R1S1)

PDB	External Database	Details
Met 1	-	initiating methionine
Asn 2	-	expression tag
Ser 3	-	expression tag
Asn 4	-	expression tag
Leu 5	-	expression tag
Pro 6	-	expression tag
Ala 7	-	expression tag
Glu 8	-	expression tag
Asn 9	-	expression tag
Leu 10	-	expression tag
Thr 11	-	expression tag
Ile 12	-	expression tag
Ser 112	Cys 114	conflict
Ile 116	Thr 118	conflict
Val 265	Met 267	conflict

B, A: 521 - 591 (UniProt: Q7Z3F1)
B, A: 592 - 870 (UniProt: G1R1S1)

PDB	External Database	Details
Asp 734	Glu 665	conflict
Thr 735	Ile 666	conflict
Ser 788	Asn 719	conflict
His 866	Arg 797	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	193989.4
Non-Polymers*	Number of molecules	14
	Total formula weight	6611.1
All*	Total formula weight	200600.4

*Water molecules are not included.