9J31

cryo-EM structure of zebrafish GPR4-Gs complex at pH 8.5

Replaces:  8ZFJ

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	366	39728.4	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
2	B (D)	scfv16	polymer	295	31560.3	1		Mus musculus
3	C (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	246	28362.0	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
4	D (R)	Soluble cytochrome b562,G-protein coupled receptor 4,lgBit	polymer	642	72372.5	1	UniProt (P0ABE7) UniProt (E7FEL0) Pfam (PF00001)	Escherichia coli	Cytochrome b-562
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7675.8	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I

Sequence modifications

B: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

A: 19 - 57 (UniProt: P63092)

PDB	External Database	Details
Gly 1	-	expression tag
Ser 2	-	expression tag
Ala 3	-	expression tag
Thr 4	-	expression tag
Val 5	-	expression tag
Ser 6	-	expression tag
Ala 7	-	expression tag
Glu 8	-	expression tag
Asp 9	-	expression tag
Lys 10	-	expression tag
Ala 11	-	expression tag
Ala 12	-	expression tag
Ala 13	-	expression tag
Glu 14	-	expression tag
Arg 15	-	expression tag
Ser 16	-	expression tag
Lys 17	-	expression tag
Met 18	-	expression tag
Asp 42	Gly 49	engineered mutation
Asn 43	Glu 50	engineered mutation
Tyr 56	Leu 63	engineered mutation

A: 198 - 377 (UniProt: P63092)

PDB	External Database	Details
Gly 58	-	linker
Gly 59	-	linker
Ser 191	-	linker
Gly 192	-	linker
Gly 193	-	linker
Ser 194	-	linker
Gly 195	-	linker
Gly 196	-	linker
Thr 197	-	linker
Asp 242	Ala 249	engineered mutation
Asp 245	Ser 252	engineered mutation
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Asp 255	Leu 272	engineered mutation
Ala 355	Ile 372	engineered mutation
Ile 358	Val 375	engineered mutation

R: -105 - 1 (UniProt: P0ABE7)

PDB	External Database	Details
Met -128	-	initiating methionine
Lys -127	-	expression tag
Thr -126	-	expression tag
Ile -125	-	expression tag
Ile -124	-	expression tag
Ala -123	-	expression tag
Leu -122	-	expression tag
Ser -121	-	expression tag
Tyr -120	-	expression tag
Ile -119	-	expression tag
Phe -118	-	expression tag
Cys -117	-	expression tag
Leu -116	-	expression tag
Val -115	-	expression tag
Phe -114	-	expression tag
Ala -113	-	expression tag
Asp -112	-	expression tag
Tyr -111	-	expression tag
Lys -110	-	expression tag
Asp -109	-	expression tag
Asp -108	-	expression tag
Asp -107	-	expression tag
Asp -106	-	expression tag
Trp -98	Met 29	engineered mutation
Ile -3	His 124	engineered mutation
Leu 1	Arg 128	engineered mutation

R: 2 - 338 (UniProt: E7FEL0)
R: 339 - 513 (PDB: 9J31)
G: 1 - 67 (UniProt: P59768)

PDB	External Database	Details
Gly 68	-	expression tag
Ser 69	-	expression tag
Ala 70	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	179699.1
All*	Total formula weight	179699.1

*Water molecules are not included.