9J1P

Cryo-EM structure of the g1:Ox-bound human GLP-1R-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas	polymer	361	41879.5	1	UniProt (P63096) UniProt (P63092) UniProt (Q5JWF2) Pfam (PF00503)	Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	345	37915.5	1	UniProt (P54311) Pfam (PF25391)	Rattus norvegicus (Norway rat)
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	70	7729.9	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (domestic cattle)
4	D (N)	Nanobody-35	polymer	140	15343.0	1		Lama glama
5	E (P)	g1:Ox	polymer	49	5109.7	1		synthetic construct
6	F (R)	Glucagon-like peptide 1 receptor	polymer	440	50860.8	1	UniProt (P43220) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (human)

Sequence modifications

A: 8 - 26 (UniProt: P63096)
A: 27 - 83 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict
Tyr 63	Leu 63	conflict

A: 84 - 204 (UniProt: P63096)
A: 205 - 253 (UniProt: P63092)

PDB	External Database	Details
Ala 226	Gly 226	conflict
Asp 249	Ala 249	conflict
Asp 252	Ser 252	conflict

A: 264 - 394 (UniProt: Q5JWF2)

PDB	External Database	Details
Asp 272	Leu 915	conflict
Ser 366	Ala 1009	conflict
Ala 372	Ile 1015	conflict
Ile 375	Val 1018	conflict

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	158838.5
All*	Total formula weight	158838.5

*Water molecules are not included.