9J0Q

Structure of mEos3.2 in the green fluorescent state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Green to red photoconvertible GFP-like protein EosFP	polymer	250	28542.2	1	UniProt (Q5S6Z9) Pfam (PF01353)	Lobophyllia hemprichii
2	B, C (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3	D (A)	water	water		18.0	245	Chemie (HOH)

Sequence modifications

A: 1 - 226 (UniProt: Q5S6Z9)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Ser -13	-	expression tag
Gln -12	-	expression tag
Asp -11	-	expression tag
Pro -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Lys 11	Asn 11	engineered mutation
5sq 64	His 62	chromophore
5sq 64	Tyr 63	chromophore
5sq 64	Gly 64	chromophore
Lys 70	Glu 70	engineered mutation
Asn 74	His 74	engineered mutation
Asn 102	Ile 102	engineered mutation
Tyr 121	His 121	engineered mutation
Thr 123	Val 123	engineered mutation
Glu 158	Thr 158	engineered mutation
Ala 189	Tyr 189	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28542.2
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	28734.4

*Water molecules are not included.