9J0F

NADH bound purinergic receptor P2Y14 in complex with Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Soluble cytochrome b562,P2Y purinoceptor 14	polymer	501	57407.7	1	UniProt (P0ABE7) UniProt (Q15391) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,P2Y14 purinergic receptor，P2Y14,G-protein coupled receptor 105,UDP-glucose receptor
5	E (R)	1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE	non-polymer		665.4	1	Chemie (NAI)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -115 - -11 (UniProt: P0ABE7)

PDB	External Database	Details
Met -162	-	initiating methionine
Lys -161	-	expression tag
Thr -160	-	expression tag
Ile -159	-	expression tag
Ile -158	-	expression tag
Ala -157	-	expression tag
Leu -156	-	expression tag
Ser -155	-	expression tag
Tyr -154	-	expression tag
Ile -153	-	expression tag
Phe -152	-	expression tag
Cys -151	-	expression tag
Leu -150	-	expression tag
Val -149	-	expression tag
Phe -148	-	expression tag
Ala -147	-	expression tag
Asp -146	-	expression tag
Tyr -145	-	expression tag
Lys -144	-	expression tag
Asp -143	-	expression tag
Asp -142	-	expression tag
Asp -141	-	expression tag
Asp -140	-	expression tag
Ala -139	-	expression tag
Gly -138	-	expression tag
Arg -137	-	expression tag
Ala -136	-	expression tag
His -135	-	expression tag
His -134	-	expression tag
His -133	-	expression tag
His -132	-	expression tag
His -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
His -126	-	expression tag
Glu -125	-	expression tag
Asn -124	-	expression tag
Leu -123	-	expression tag
Tyr -122	-	expression tag
Phe -121	-	expression tag
Gln -120	-	expression tag
Ser -119	-	expression tag
Gly -118	-	expression tag
Ala -117	-	expression tag
Pro -116	-	expression tag
Trp -109	Met 29	conflict
Ile -14	His 124	conflict
Leu -10	-	linker
Glu -9	-	linker
Phe -8	-	linker
Leu -7	-	linker
Glu -6	-	linker
Val -5	-	linker
Leu -4	-	linker
Phe -3	-	linker
Gln -2	-	linker
Gly -1	-	linker
Pro 0	-	linker
Ala 1	-	linker

R: 2 - 338 (UniProt: Q15391)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	146739.8
Non-Polymers*	Number of molecules	1
	Total formula weight	665.4
All*	Total formula weight	147405.2

*Water molecules are not included.