9J0B

UDP-Glucose bound purinergic receptor P2Y14 in complex with Gi

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Soluble cytochrome b562,P2Y purinoceptor 14	polymer	501	57407.7	1	UniProt (P0ABE7) UniProt (Q15391) Pfam (PF07361) Pfam (PF00001)	Escherichia coli	Cytochrome b-562,P2Y14 purinergic receptor，P2Y14,G-protein coupled receptor 105,UDP-glucose receptor
5	E (S)	ScFv16	polymer	297	31870.6	1		Homo sapiens (human)
6	F (R)	URIDINE-5'-DIPHOSPHATE-GLUCOSE	non-polymer		566.3	1	Chemie (UPG)

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

R: -115 - -11 (UniProt: P0ABE7)

PDB	External Database	Details
Met -162	-	initiating methionine
Lys -161	-	expression tag
Thr -160	-	expression tag
Ile -159	-	expression tag
Ile -158	-	expression tag
Ala -157	-	expression tag
Leu -156	-	expression tag
Ser -155	-	expression tag
Tyr -154	-	expression tag
Ile -153	-	expression tag
Phe -152	-	expression tag
Cys -151	-	expression tag
Leu -150	-	expression tag
Val -149	-	expression tag
Phe -148	-	expression tag
Ala -147	-	expression tag
Asp -146	-	expression tag
Tyr -145	-	expression tag
Lys -144	-	expression tag
Asp -143	-	expression tag
Asp -142	-	expression tag
Asp -141	-	expression tag
Asp -140	-	expression tag
Ala -139	-	expression tag
Gly -138	-	expression tag
Arg -137	-	expression tag
Ala -136	-	expression tag
His -135	-	expression tag
His -134	-	expression tag
His -133	-	expression tag
His -132	-	expression tag
His -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
His -126	-	expression tag
Glu -125	-	expression tag
Asn -124	-	expression tag
Leu -123	-	expression tag
Tyr -122	-	expression tag
Phe -121	-	expression tag
Gln -120	-	expression tag
Ser -119	-	expression tag
Gly -118	-	expression tag
Ala -117	-	expression tag
Pro -116	-	expression tag
Trp -109	Met 29	conflict
Ile -14	His 124	conflict
Leu -10	-	linker
Glu -9	-	linker
Phe -8	-	linker
Leu -7	-	linker
Glu -6	-	linker
Val -5	-	linker
Leu -4	-	linker
Phe -3	-	linker
Gln -2	-	linker
Gly -1	-	linker
Pro 0	-	linker
Ala 1	-	linker

R: 2 - 338 (UniProt: Q15391)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	178610.4
Non-Polymers*	Number of molecules	1
	Total formula weight	566.3
All*	Total formula weight	179176.7

*Water molecules are not included.