9IZ4

Crystal structure of phosphonopyruvate decarboxylase RhiEF from Bacillus subtilis ATCC6633 in complex with thiamine pyrophosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G, I... (A, C, E, G, I...)	Putative phosphonopyruvate decarboxylase alpha subunit	polymer	181	20342.2	6	UniProt (D4HRI2) Pfam (PF02776)	Bacillus spizizenii ATCC 6633 = JCM 2499
2	B, D, F, H, J... (B, D, F, H, J...)	Putative phosphonopyruvate decarboxylase beta subunit	polymer	186	20567.5	6	UniProt (D4HRI3) Pfam (PF02775)	Bacillus spizizenii ATCC 6633 = JCM 2499
3	M, O, R, U, W... (B, D, F, H, J...)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
4	AA, N, P, S, V... (L, B, D, F, H...)	THIAMINE DIPHOSPHATE	non-polymer		425.3	6	Chemie (TPP)
5	Q, T, Y (E, G, K)	SULFOPYRUVATE	non-polymer		168.1	3	Chemie (SPV)

Sequence modifications

A, C, E, G, I, K: 1 - 167 (UniProt: D4HRI2)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	245458.3
Non-Polymers*	Number of molecules	15
	Total formula weight	3202.1
All*	Total formula weight	248660.4

*Water molecules are not included.