9IYH

Structure of Phosphopantetheine adenylyltransferase (PPAT) from Enterobacter spp. with the expression tag bound in the substrate binding site of a neighbouring molecule at 2.25 A resolution.

Replaces:  8I8L

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Phosphopantetheine adenylyltransferase	polymer	173	19182.3	6	UniProt (A4W515) Pfam (PF01467)	Enterobacter sp. 638	Dephospho-CoA pyrophosphorylase,Pantetheine-phosphate adenylyltransferase,PPAT
2	G, I, K, L, N... (A, B, C, D, E...)	PHOSPHONOACETIC ACID	non-polymer		140.0	6	Chemie (PAE)
3	H, M (B, D)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	J, P (C, F)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	Q, R, S, T, U... (A, B, C, D, E...)	water	water		18.0	518	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 159 (UniProt: A4W515)

PDB	External Database	Details
Met -13	-	initiating methionine
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	115093.6
Non-Polymers*	Number of molecules	10
	Total formula weight	1148.5
All*	Total formula weight	116242.1

*Water molecules are not included.