9IYB
Cryo-EM Structure of the Prostaglandin D2 Receptor 2-PGD2 Coupled to G Protein
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Prostaglandin D2 receptor 2 | polymer | 395 | 43319.7 | 1 | UniProt (Q9Y5Y4) Pfam (PF00001) | Homo sapiens (human) | Chemoattractant receptor-homologous molecule expressed on Th2 cells,G-protein coupled receptor 44 |
| 2 | B (B) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | D (D) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | E (E) | scFv16 | polymer | 248 | 26408.5 | 1 | Rattus norvegicus | ||
| 6 | F (A) | [(2~{R})-1-hexadecanoyloxy-3-[oxidanyl-[(2~{S},3~{R},5~{R},6~{S})-2,3,4,5,6-pentakis(oxidanyl)cyclohexyl]oxy-phosphoryl]oxy-propan-2-yl] (~{Z})-octadec-9-enoate | non-polymer | 837.1 | 1 | Chemie (A1D5Q) | |||
| 7 | G (A) | PROSTAGLANDIN D2 | non-polymer | 352.5 | 1 | Chemie (PG2) | |||
| 8 | H (A) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
B: 1 - 354 (UniProt: P63096)
C: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 155918.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 1189.5 | |
| All* | Total formula weight | 157108.4 |
