9IXL
Crystal structure of the CYP153A double mutant L354T/V456G from Marinobacter aquaeolei
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (B, A) | Cytochrome P450 | polymer | 480 | 55224.9 | 2 | UniProt (A0A368UNN3) Pfam (PF00067) | Marinobacter nauticus (Marinobacter hydrocarbonoclasticus Marinobacter aquaeolei) | CYP153A |
| 2 | C, G (B, A) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
| 3 | D, H (B, A) | LAURIC ACID | non-polymer | 200.3 | 2 | Chemie (DAO) | |||
| 4 | E, F, I, J, K (B, A) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 5 | L, M (B, A) | water | water | 18.0 | 356 | Chemie (HOH) |
Sequence modifications
B, A: 5 - 474 (UniProt: A0A368UNN3)
| PDB | External Database | Details |
|---|---|---|
| Met 3 | - | initiating methionine |
| Gly 4 | - | expression tag |
| Thr 358 | Leu 354 | engineered mutation |
| Gly 460 | Val 456 | engineered mutation |
| Leu 475 | - | expression tag |
| Glu 476 | - | expression tag |
| His 477 | - | expression tag |
| His 478 | - | expression tag |
| His 479 | - | expression tag |
| His 480 | - | expression tag |
| His 481 | - | expression tag |
| His 482 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 110449.8 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 2094.1 | |
| All* | Total formula weight | 112543.9 |
