9IVN
Crystal structure of KRED mutant-Y199A/N149L
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, F, G...) | Short-chain dehydrogenase/reductase SDR | polymer | 259 | 26736.7 | 8 | UniProt (A4XEP2) Pfam (PF13561) | Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CCUG 56034 / CIP 105152 / NBRC 16084 / F199) | |
| 2 | CA, FA, I, M, P... (D, E, A, B, C...) | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 8 | Chemie (NAD) | |||
| 3 | AA, DA, GA, J, N... (H, D, E, A, B...) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 8 | Chemie (PEG) | |||
| 4 | EA, IA, JA, K (D, E, A) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 4 | Chemie (DMS) | |||
| 5 | L (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | BA, O, Y (H, B, G) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 7 | HA, R, U, V (E, C, F) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 8 | KA, LA, MA, NA, OA... (A, B, C, F, G...) | water | water | 18.0 | 277 | Chemie (HOH) |
Sequence modifications
A, B, C, F, G, H, D, E: 1 - 259 (UniProt: A4XEP2)
| PDB | External Database | Details |
|---|---|---|
| Leu 149 | Asn 149 | engineered mutation |
| Ala 199 | Tyr 199 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 213893.9 | |
| Non-Polymers* | Number of molecules | 28 |
| Total formula weight | 7097.4 | |
| All* | Total formula weight | 220991.4 |
