9IVC

Cryo-EM structure of AbA-bound Aur1-Kei1 complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Inositol phosphorylceramide synthase catalytic subunit AUR1	polymer	449	49956.9	2	UniProt (P36107)	Saccharomyces cerevisiae S288C	IPC synthase catalytic subunit AUR1,Aureobasidin A resistance protein,Phosphatidylinositol:ceramide phosphoinositol transferase
2	B, D (B, D)	Inositol phosphorylceramide synthase regulatory subunit KEI1	polymer	242	27777.1	2	UniProt (Q06346)	Saccharomyces cerevisiae S288C	ICP synthase regulatory subunit KEI1,KEX2-cleavable protein essential for inositol phosphorylceramide synthesis
3	E, F (E, F)	Aureobasidin A	polymer	9	1119.4	2		Aureobasidium pullulans R106

Sequence modifications

A, C: 1 - 401 (UniProt: P36107)

PDB	External Database	Details
Met -47	-	initiating methionine
Ala -46	-	expression tag
Ser -45	-	expression tag
Gly -44	-	expression tag
His -43	-	expression tag
Ser -42	-	expression tag
Ala -41	-	expression tag
Trp -40	-	expression tag
Ser -39	-	expression tag
His -38	-	expression tag
Pro -37	-	expression tag
Gln -36	-	expression tag
Phe -35	-	expression tag
Glu -34	-	expression tag
Lys -33	-	expression tag
Gly -32	-	expression tag
Gly -31	-	expression tag
Gly -30	-	expression tag
Ser -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Ala -21	-	expression tag
Trp -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
Pro -17	-	expression tag
Gln -16	-	expression tag
Phe -15	-	expression tag
Glu -14	-	expression tag
Lys -13	-	expression tag
Val -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Asp -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Asp -6	-	expression tag
Ala -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

B, D: 1 - 221 (UniProt: Q06346)

PDB	External Database	Details
Met -20	-	initiating methionine
Ala -19	-	expression tag
Asp -18	-	expression tag
Tyr -17	-	expression tag
Lys -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Lys -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Pro -8	-	expression tag
Asp -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Asp -4	-	expression tag
Ala -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	157707.0
All*	Total formula weight	157707.0

*Water molecules are not included.