9IUZ

Constitutively active mutant(Y276H) of Arabidopsis phytochrome B(phyB) in complex with phytochrome-interacting factor 6(PIF6)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, A)	Phytochrome B	polymer	913	99764.9	2	UniProt (P14713) Pfam (PF08446) Pfam (PF01590) Pfam (PF00360) Pfam (PF00989)	Arabidopsis thaliana (thale cress)	Protein LONG HYPOCOTYL 3,Protein OUT OF PHASE 1
2	C (C)	Phytochrome-interacting factor 6	polymer	105	11688.1	1	UniProt (Q8L5W7)	Arabidopsis thaliana (thale cress)	PIF6, Transcription factor PIF6, Basic helix-loop-helix protein 132,AtbHLH132,bHLH 132,Phytochrome interacting factor 3-like 2,Transcription factor EN 111,bHLH transcription factor bHLH132
3	D, E (B, A)	3-[5-[[(3~{R},4~{R})-3-ethyl-4-methyl-5-oxidanylidene-3,4-dihydropyrrol-2-yl]methyl]-2-[[5-[(4-ethyl-3-methyl-5-oxidanylidene-pyrrol-2-yl)methyl]-3-(3-hydroxy-3-oxopropyl)-4-methyl-1~{H}-pyrrol-2-yl]methyl]-4-methyl-1~{H}-pyrrol-3-yl]propanoic acid	non-polymer		586.7	2	Chemie (O6E)

Sequence modifications

B, A: 1 - 908 (UniProt: P14713)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
His 276	Tyr 276	engineered mutation

C: 1 - 100 (UniProt: Q8L5W7)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Leu -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	211217.9
Non-Polymers*	Number of molecules	2
	Total formula weight	1173.4
All*	Total formula weight	212391.2

*Water molecules are not included.