9ITG

Cryo-EM structure of human XPR1-E622A/F623A mutant in complex with InsP6 in inward-facing state in the presence of 10 mM KH2PO4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Solute carrier family 53 member 1	polymer	704	82539.9	2	UniProt (Q9UBH6) Pfam (PF03105) Pfam (PF03124)	Homo sapiens (human)	Phosphate exporter SLC53A1,Protein SYG1 homolog,Xenotropic and polytropic murine leukemia virus receptor X3,X-receptor,Xenotropic and polytropic retrovirus receptor 1
2	C, K (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
3	D, L (A, B)	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	2	Chemie (IHP)
4	E, M (A, B)	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
5	F, G, N, O (A, B)	1,2-Distearoyl-sn-glycerophosphoethanolamine	non-polymer		748.1	4	Chemie (3PE)
6	H, P (A, B)	CHOLESTEROL HEMISUCCINATE	non-polymer		486.7	2	Chemie (Y01)
7	I, Q (A, B)	PALMITIC ACID	non-polymer		256.4	2	Chemie (PLM)
8	J, R (A, B)	(2S,3R,4E)-3-hydroxy-2-(octanoylamino)octadec-4-en-1-yl dihydrogen phosphate	non-polymer		505.7	2	Chemie (1T9)
9	S, T (A, B)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

A, B: 1 - 696 (UniProt: Q9UBH6)

PDB	External Database	Details
Ala 622	Glu 622	engineered mutation
Ala 623	Phe 623	engineered mutation
Ser 697	-	expression tag
Arg 698	-	expression tag
Glu 699	-	expression tag
Asn 700	-	expression tag
Leu 701	-	expression tag
Tyr 702	-	expression tag
Phe 703	-	expression tag
Gln 704	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	165079.7
Non-Polymers*	Number of molecules	16
	Total formula weight	7773.2
All*	Total formula weight	172852.9

*Water molecules are not included.