9ISZ

Structure of Clr4 catalyzing K14-ubiquitinated histone H3 K9 methylation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Histone-lysine N-methyltransferase, H3 lysine-9 specific	polymer	301	34078.1	2	UniProt (O60016) Pfam (PF05033) Pfam (PF00856)	Schizosaccharomyces pombe 972h-	Cryptic loci regulator 4,Histone H3-K9 methyltransferase,H3-K9-HMTase,HKMT,Lysine N-methyltransferase 1,Protein lysine methyltransferase clr4,PKMT
2	C, D (C, D)	Ubiquitin	polymer	76	8622.9	2	UniProt (P0CG47) Pfam (PF00240) UniProt (by SIFTS) (P0CG48)	Homo sapiens (human)
3	E, F (E, F)	Histone H3.1/H3.2	polymer	17	1807.1	2	UniProt (P09988)	Schizosaccharomyces pombe 972h-
4	G, L (A, B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)
5	H, I, J, K, M... (A, B)	ZINC ION	non-polymer		65.4	8	Chemie (ZN)
6	Q, R, S, T, U (A, B, C, D, F)	water	water		18.0	53	Chemie (HOH)

Sequence modifications

A, B: 191 - 490 (UniProt: O60016)

PDB	External Database	Details
Gly 190	-	expression tag

C, D: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Cys 76	Gly 228	engineered mutation

E, F: 3 - 19 (UniProt: P09988)

PDB	External Database	Details
Nle 9	Lys 10	engineered mutation
Cys 14	Lys 15	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	89016.1
Non-Polymers*	Number of molecules	10
	Total formula weight	1320.1
All*	Total formula weight	90336.3

*Water molecules are not included.