9ISS
Crystal Structure of Cytochrome P450BM3 III-10C1 Mutant Heme Domain with N-Decanoyl-L-Homoserine Lactone
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Bifunctional cytochrome P450/NADPH--P450 reductase | polymer | 455 | 52222.5 | 2 | UniProt (P14779) | Priestia megaterium | Cytochrome P450(BM-3),Cytochrome P450BM-3,Fatty acid monooxygenase,Flavocytochrome P450 BM3 |
| 2 | C, H (A, B) | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 2 | Chemie (HEM) | |||
| 3 | D, I (A, B) | N-[(3S)-2-oxotetrahydrofuran-3-yl]decanamide | non-polymer | 255.4 | 2 | Chemie (HL0) | |||
| 4 | E, J (A, B) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 2 | Chemie (DMS) | |||
| 5 | F, G, K, L (A, B) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 6 | M, N (A, B) | water | water | 18.0 | 611 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 455 (UniProt: P14779)
| PDB | External Database | Details |
|---|---|---|
| Trp 47 | Arg 48 | engineered mutation |
| Leu 49 | Thr 50 | engineered mutation |
| Val 74 | Ala 75 | engineered mutation |
| Pro 188 | Leu 189 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 104445.1 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2268.3 | |
| All* | Total formula weight | 106713.4 |
