9IS8

Cryo-EM structure of AKT1-AtKC1(G315D)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D (A, C, D)	Potassium channel AKT1	polymer	885	99812.3	3	UniProt (Q38998) Pfam (PF00520) Pfam (PF00027) Pfam (PF00023) Pfam (PF11834)	Arabidopsis thaliana (thale cress)
2	B (B)	Potassium channel KAT3	polymer	710	80394.9	1	UniProt (P92960) Pfam (PF00520) Pfam (PF00027) Pfam (PF11834)	Arabidopsis thaliana (thale cress)	AKT4,AtKC1,Potassium channel TKC
3	E, F, G, J (A, B, C, D)	(2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate	non-polymer		760.1	4	Chemie (POV)
4	H, I, K (C, D)	POTASSIUM ION	non-polymer		39.1	3	Chemie (K) PubChem (813)

Sequence modifications

A, C, D: 1 - 857 (UniProt: Q38998)

PDB	External Database	Details
Leu 858	-	expression tag
Glu 859	-	expression tag
Gly 860	-	expression tag
Ser 861	-	expression tag
Asp 862	-	expression tag
Glu 863	-	expression tag
Val 864	-	expression tag
Asp 865	-	expression tag
Ala 866	-	expression tag
Gly 867	-	expression tag
Ser 868	-	expression tag
Ala 869	-	expression tag
Ala 870	-	expression tag
Ala 871	-	expression tag
Ser 872	-	expression tag
Gly 873	-	expression tag
Gly 874	-	expression tag
Ser 875	-	expression tag
Gly 876	-	expression tag
Ser 877	-	expression tag
Asp 878	-	expression tag
Tyr 879	-	expression tag
Lys 880	-	expression tag
Asp 881	-	expression tag
Asp 882	-	expression tag
Asp 883	-	expression tag
Asp 884	-	expression tag
Lys 885	-	expression tag

B: 1 - 662 (UniProt: P92960)

PDB	External Database	Details
Asp 315	Gly 315	engineered mutation
Leu 663	-	expression tag
Glu 664	-	expression tag
Gly 665	-	expression tag
Ser 666	-	expression tag
Asp 667	-	expression tag
Glu 668	-	expression tag
Val 669	-	expression tag
Asp 670	-	expression tag
Ala 671	-	expression tag
Gly 672	-	expression tag
Ser 673	-	expression tag
Ala 674	-	expression tag
Ala 675	-	expression tag
Ala 676	-	expression tag
Ser 677	-	expression tag
Gly 678	-	expression tag
Gly 679	-	expression tag
Ser 680	-	expression tag
Gly 681	-	expression tag
Ser 682	-	expression tag
Trp 683	-	expression tag
Ser 684	-	expression tag
His 685	-	expression tag
Pro 686	-	expression tag
Gln 687	-	expression tag
Phe 688	-	expression tag
Glu 689	-	expression tag
Lys 690	-	expression tag
Gly 691	-	expression tag
Gly 692	-	expression tag
Gly 693	-	expression tag
Ala 694	-	expression tag
Arg 695	-	expression tag
Gly 696	-	expression tag
Gly 697	-	expression tag
Ser 698	-	expression tag
Gly 699	-	expression tag
Gly 700	-	expression tag
Gly 701	-	expression tag
Ser 702	-	expression tag
Trp 703	-	expression tag
Ser 704	-	expression tag
His 705	-	expression tag
Pro 706	-	expression tag
Gln 707	-	expression tag
Phe 708	-	expression tag
Glu 709	-	expression tag
Lys 710	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	379831.7
Non-Polymers*	Number of molecules	7
	Total formula weight	3157.6
All*	Total formula weight	382989.3

*Water molecules are not included.