9IP3

Cryo-EM structure of the RNA-dependent RNA polymerase complex in a compact conformation from Ebola virus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose/maltodextrin-binding periplasmic protein,RNA-directed RNA polymerase L	polymer	1839	207335.2	1	UniProt (P0AEX9) UniProt (Q05318) Pfam (PF00946) Pfam (PF14318)	Escherichia coli (strain K12)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Protein L,Large structural protein,Replicase,Transcriptase
2	B, C, D, E (B, C, D, E)	Maltose/maltodextrin-binding periplasmic protein,Polymerase cofactor VP35	polymer	657	72190.3	4	UniProt (P0AEX9) UniProt (Q05127) Pfam (PF02097)	Escherichia coli (strain K12)	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,Ebola VP35,eVP35
3	F (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: -382 - -19 (UniProt: P0AEX9)

PDB	External Database	Details
Met -428	-	initiating methionine
Gly -427	-	expression tag
Ser -426	-	expression tag
Gly -425	-	expression tag
Trp -424	-	expression tag
Ser -423	-	expression tag
His -422	-	expression tag
Pro -421	-	expression tag
Gln -420	-	expression tag
Phe -419	-	expression tag
Glu -418	-	expression tag
Lys -417	-	expression tag
Gly -416	-	expression tag
Gly -415	-	expression tag
Gly -414	-	expression tag
Ser -413	-	expression tag
Gly -412	-	expression tag
Gly -411	-	expression tag
Gly -410	-	expression tag
Ser -409	-	expression tag
Gly -408	-	expression tag
Gly -407	-	expression tag
Ser -406	-	expression tag
Ala -405	-	expression tag
Trp -404	-	expression tag
Ser -403	-	expression tag
His -402	-	expression tag
Pro -401	-	expression tag
Gln -400	-	expression tag
Phe -399	-	expression tag
Glu -398	-	expression tag
Lys -397	-	expression tag
Gly -396	-	expression tag
Ser -395	-	expression tag
Ala -394	-	expression tag
Ser -393	-	expression tag
His -392	-	expression tag
His -391	-	expression tag
His -390	-	expression tag
His -389	-	expression tag
His -388	-	expression tag
His -387	-	expression tag
Gly -386	-	expression tag
Thr -385	-	expression tag
Lys -384	-	expression tag
Thr -383	-	expression tag
Gly -18	-	linker
Thr -17	-	linker
Asp -16	-	linker
Tyr -15	-	linker
Asp -14	-	linker
Ile -13	-	linker
Pro -12	-	linker
Thr -11	-	linker
Thr -10	-	linker
Leu -9	-	linker
Glu -8	-	linker
Val -7	-	linker
Leu -6	-	linker
Phe -5	-	linker
Gln -4	-	linker
Gly -3	-	linker
Pro -2	-	linker
Gly -1	-	linker
Ser 0	-	linker

A: 1 - 1400 (UniProt: Q05318)

PDB	External Database	Details
Ser 1401	-	expression tag
Arg 1402	-	expression tag
Asp 1403	-	expression tag
Tyr 1404	-	expression tag
Lys 1405	-	expression tag
Asp 1406	-	expression tag
Asp 1407	-	expression tag
Asp 1408	-	expression tag
Asp 1409	-	expression tag
Lys 1410	-	expression tag

B, C, D, E: -302 - 61 (UniProt: P0AEX9)

PDB	External Database	Details
Met -316	-	initiating methionine
Gly -315	-	expression tag
Ser -314	-	expression tag
Ser -313	-	expression tag
His -312	-	expression tag
His -311	-	expression tag
His -310	-	expression tag
His -309	-	expression tag
His -308	-	expression tag
His -307	-	expression tag
Gly -306	-	expression tag
Thr -305	-	expression tag
Lys -304	-	expression tag
Thr -303	-	expression tag
Gly 62	-	linker
Thr 63	-	linker
Asp 64	-	linker
Tyr 65	-	linker
Asp 66	-	linker
Ile 67	-	linker
Pro 68	-	linker
Thr 69	-	linker
Thr 70	-	linker
Glu 71	-	linker
Asn 72	-	linker
Leu 73	-	linker
Tyr 74	-	linker
Phe 75	-	linker
Gln 76	-	linker
Gly 77	-	linker
Gly 78	-	linker
Ser 79	-	linker

B, C, D, E: 80 - 340 (UniProt: Q05127)

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	496096.6
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	496162.0

*Water molecules are not included.