9II1
GH57 family Amylopullulanase mutant E256L from Aquifex aeolicus in complex with maltohexaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Glycoside hydrolase family 57 N-terminal domain-containing protein | polymer | 477 | 57237.3 | 2 | UniProt (O66934) Pfam (PF03065) | Aquifex aeolicus (strain VF5) | |
| 2 | C, E (C, E) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 828.7 | 2 | In PDB BIRD (PRD_900030) GlyTouCan (G50146AM) | |||
| 3 | D (D) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 666.6 | 1 | In PDB BIRD (PRD_900010) GlyTouCan (G87171PZ) | |||
| 4 | F (G) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 504.4 | 1 | In PDB BIRD (PRD_900065) GlyTouCan (G00395FS) | |||
| 5 | G, H, I, J, K (A, B) | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
| 6 | L, M (A, B) | water | water | 18.0 | 549 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 477 (UniProt: O66934)
| PDB | External Database | Details |
|---|---|---|
| Leu 256 | Glu 256 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 114474.6 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 2828.5 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 460.5 | |
| All* | Total formula weight | 117763.5 |
