9IG7
KOD-H4 DNA polymerase mutant in a binary complex with DNA:DNA containing two AtNA nucleotides
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase | polymer | 774 | 89976.5 | 1 | UniProt (D0VWU9) | Thermococcus kodakarensis KOD1 | |
| 2 | B (P) | DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*CP*AP*CP*A)-3') | polymer | 13 | 3930.6 | 1 | synthetic construct | ||
| 3 | C (T) | DNA/RNA (5'-D(*AP*AP*C)-R(P*(A6U)P*(A6G))-D(P*TP*GP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3') | polymer | 16 | 4976.2 | 1 | synthetic construct | ||
| 4 | D (A) | IMIDAZOLE | non-polymer | 69.1 | 1 | Chemie (IMD) | |||
| 5 | E, G, J (A) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 6 | F (A) | FORMIC ACID | non-polymer | 46.0 | 1 | Chemie (FMT) | |||
| 7 | H (A) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
| 8 | I, L (A, T) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 9 | K (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 10 | M (T) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 11 | N, O, P (A, P, T) | water | water | 18.0 | 140 | Chemie (HOH) |
Sequence modifications
A: 1 - 774 (UniProt: D0VWU9)
| PDB | External Database | Details |
|---|---|---|
| Gln 93 | Val 93 | engineered mutation |
| Thr 114 | Ile 114 | engineered mutation |
| Ala 141 | Asp 141 | engineered mutation |
| Ala 143 | Glu 143 | engineered mutation |
| His 147 | Glu 147 | engineered mutation |
| Lys 383 | Ser 383 | engineered mutation |
| Gly 429 | Lys 429 | engineered mutation |
| Leu 445 | Phe 445 | engineered mutation |
| Leu 485 | Ala 485 | engineered mutation |
| Val 493 | Tyr 493 | engineered mutation |
| His 496 | Tyr 496 | engineered mutation |
| Met 497 | Tyr 497 | engineered mutation |
| Phe 499 | Tyr 499 | engineered mutation |
| Glu 500 | Ala 500 | engineered mutation |
| Asn 501 | Arg 501 | engineered mutation |
| Leu 521 | Ile 521 | engineered mutation |
| Lys 584 | Glu 584 | engineered mutation |
| Lys 664 | Glu 664 | engineered mutation |
| Arg 726 | Lys 726 | engineered mutation |
| Lys 735 | Asn 735 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 98883.3 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 790.0 | |
| All* | Total formula weight | 99673.3 |
