9IFB

Crystal structure of S-adenosyl-L-homocysteine hydrolase mutant C221W from Pyrococcus furiosus in complex with inosine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Adenosylhomocysteinase	polymer	441	49703.3	2	UniProt (P50251) Pfam (PF05221) Pfam (PF00670)	Pyrococcus furiosus	S-adenosyl-L-homocysteine hydrolase,AdoHcyase
2	C, D, E, F (A, B)	INOSINE	non-polymer		268.2	4	Chemie (NOS)
3	G, H (A, B)	water	water		18.0	161	Chemie (HOH)

Sequence modifications

A, B: 1 - 421 (UniProt: P50251)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Trp 221	Cys 221	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	99406.7
Non-Polymers*	Number of molecules	4
	Total formula weight	1072.9
All*	Total formula weight	100479.6

*Water molecules are not included.