9IBT
Branching Sucrase Brs-B in complex with isomaltopentaose in the active site (alternative conformation)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | dextransucrase | polymer | 1183 | 132408.6 | 2 | UniProt (A0A5A5U0V5) | Leuconostoc citreum | Dextransucrase,Sucrose 6-glucosyltransferase |
| 2 | C, D (C, D) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 504.4 | 2 | In PDB GlyTouCan (G02998EX) | |||
| 3 | E, F, J (E, Z, R) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 666.6 | 3 | In PDB GlyTouCan (G80662SX) | |||
| 4 | G (X) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 828.7 | 1 | In PDB GlyTouCan (G09161MH) | |||
| 5 | H, I (K, T) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 1477.3 | 2 | In PDB | |||
| 6 | K (P) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB GlyTouCan (G69864PN) | |||
| 7 | L, M (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) PubChem (271) | |||
| 8 | N, O (A, B) | water | water | 18.0 | 11 | Chemie (HOH) |
Sequence modifications
A, B: 154 - 1313 (UniProt: A0A5A5U0V5)
| PDB | External Database | Details |
|---|---|---|
| Met 151 | - | initiating methionine |
| Ala 152 | - | expression tag |
| Ser 153 | - | expression tag |
| Gln 709 | Glu 709 | conflict |
| Ser 768 | Phe 768 | conflict |
| Thr 1103 | Ala 1103 | conflict |
| Lys 1314 | - | expression tag |
| Gly 1315 | - | expression tag |
| Gly 1316 | - | expression tag |
| Arg 1317 | - | expression tag |
| Ala 1318 | - | expression tag |
| Asp 1319 | - | expression tag |
| Pro 1320 | - | expression tag |
| Ala 1321 | - | expression tag |
| Phe 1322 | - | expression tag |
| Leu 1323 | - | expression tag |
| Tyr 1324 | - | expression tag |
| Lys 1325 | - | expression tag |
| Val 1326 | - | expression tag |
| Val 1327 | - | expression tag |
| His 1328 | - | expression tag |
| His 1329 | - | expression tag |
| His 1330 | - | expression tag |
| His 1331 | - | expression tag |
| His 1332 | - | expression tag |
| His 1333 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 264817.2 | |
| Branched | Number of molecules | 9 |
| Total formula weight | 7134.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 80.2 | |
| All* | Total formula weight | 272031.5 |
