9I9I

Cryo-EM structure of CAK-CDK11

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (K)	Isoform 7 of Cyclin-dependent kinase 11B	polymer	442	49970.6	1	UniProt (P21127) Pfam (PF00069)	Homo sapiens (human)	Cell division cycle 2-like protein kinase 1,CLK-1,Cell division protein kinase 11B,Galactosyltransferase-associated protein kinase p58/GTA,PITSLRE serine/threonine-protein kinase CDC2L1,p58 CLK-1
2	B (H)	CDK-activating kinase assembly factor MAT1	polymer	328	38132.3	1	UniProt (P51948) Pfam (PF17121) Pfam (PF06391)	Homo sapiens (human)	CDK7/cyclin-H assembly factor,Cyclin-G1-interacting protein,Menage a trois,RING finger protein 66,RING finger protein MAT1,p35,p36
3	C (I)	Cyclin-H	polymer	324	37721.5	1	UniProt (P51946) Pfam (PF00134) Pfam (PF16899)	Homo sapiens (human)	MO15-associated protein,p34,p37
4	D (J)	Cyclin-dependent kinase 7	polymer	391	43651.1	1	UniProt (P50613) Pfam (PF00069)	Homo sapiens (human)	39 kDa protein kinase,p39 Mo15,CDK-activating kinase 1,Cell division protein kinase 7,Serine/threonine-protein kinase 1,TFIIH basal transcription factor complex kinase subunit
5	E, G (K, J)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
6	F, H (K, J)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	I (J)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

K: 357 - 795 (UniProt: P21127)

PDB	External Database	Details
Ser 354	-	expression tag
Asn 355	-	expression tag
Ala 356	-	expression tag

H: 1 - 309 (UniProt: P51948)

PDB	External Database	Details
Met -18	-	initiating methionine
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

I: 1 - 323 (UniProt: P51946)

PDB	External Database	Details
Ace 0	-	acetylation

J: 1 - 346 (UniProt: P50613)

PDB	External Database	Details
Met -44	-	initiating methionine
Ala -43	-	expression tag
Ser -42	-	expression tag
Trp -41	-	expression tag
Ser -40	-	expression tag
His -39	-	expression tag
Pro -38	-	expression tag
Gln -37	-	expression tag
Phe -36	-	expression tag
Glu -35	-	expression tag
Lys -34	-	expression tag
Gly -33	-	expression tag
Gly -32	-	expression tag
Gly -31	-	expression tag
Ser -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Trp -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
Pro -18	-	expression tag
Gln -17	-	expression tag
Phe -16	-	expression tag
Glu -15	-	expression tag
Lys -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Gly -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	169475.5
Non-Polymers*	Number of molecules	4
	Total formula weight	903.0
All*	Total formula weight	170378.5

*Water molecules are not included.