9I8Y
SpCas12Cas12f1 in complex with sgRNA and cognate DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | CRISPR-associated endodeoxyribonuclease Cas12f1 | polymer | 500 | 57259.9 | 2 | UniProt (P0DW62) | Syntrophomonas palmitatica JCM 14374 | SpCas12f1,CRISPR-associated endonuclease C2c10 |
| 2 | C (C) | DNA target strand | polymer | 32 | 9891.4 | 1 | synthetic construct | ||
| 3 | D (D) | DNA non-target strand | polymer | 19 | 5795.8 | 1 | synthetic construct | ||
| 4 | E (E) | sgRNA (single-guide RNA) | polymer | 210 | 67845.4 | 1 | synthetic construct | ||
| 5 | F, G, H (A, B) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) |
Sequence modifications
A, B: 1 - 497 (UniProt: P0DW62)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 198052.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 196.2 | |
| All* | Total formula weight | 198248.5 |
