9I4R

N-terminal Oic streptag II in Sav E44V-S45T-V47R-D67A-K121R variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (B, C, D, E)	Streptavidin	polymer	158	16462.9	4	UniProt (P22629)	Streptomyces avidinii
2	E, F, G, H (F, G, H, I)	Oic-P-streptagII	polymer	10	1308.5	4		synthetic construct
3	I (E)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	J, K, L, M, N... (B, C, D, E, F...)	water	water		18.0	140	Chemie (HOH)

Sequence modifications

B, C, D, E: 15 - 159 (UniProt: P22629)

PDB	External Database	Details
Ala 2	-	expression tag
Ser 3	-	expression tag
Met 4	-	expression tag
Thr 5	-	expression tag
Gly 6	-	expression tag
Gly 7	-	expression tag
Gln 8	-	expression tag
Gln 9	-	expression tag
Met 10	-	expression tag
Gly 11	-	expression tag
Arg 12	-	expression tag
Asp 13	-	expression tag
Gln 14	-	expression tag
Val 44	Glu 68	engineered mutation
Thr 45	Ser 69	engineered mutation
Arg 47	Val 71	engineered mutation
Ala 67	Asp 91	engineered mutation
Arg 121	Lys 145	engineered mutation
Gln 134	Lys 158	conflict

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	71085.5
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	71177.6

*Water molecules are not included.