9I49

Cobalamin-binding chimera 10 (Cob10) (crystallization condition 1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Coenzyme B12-dependent mutase,Uroporphyrinogen-III synthase	polymer	286	31493.5	1	UniProt (Q9YBB1) UniProt (P10746)	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	UROIIIS,UROS,Hydroxymethylbilane hydrolyase [cyclizing],Uroporphyrinogen-III cosynthase
2	B (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA) PubChem (923)
3	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
4	D (A)	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
5	E (A)	water	water		18.0	119	Chemie (HOH)

Sequence modifications

A: 2 - 36 (UniProt: Q9YBB1)

PDB	External Database	Details
Met 1	-	initiating methionine

A: 37 - 181 (UniProt: P10746)

PDB	External Database	Details
His 153	Arg 148	engineered mutation
Ala 182	-	linker
Gly 183	-	linker
Asn 184	-	linker

A: 185 - 278 (UniProt: Q9YBB1)

PDB	External Database	Details
Leu 279	-	expression tag
Glu 280	-	expression tag
His 281	-	expression tag
His 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	31493.5
Non-Polymers*	Number of molecules	3
	Total formula weight	127.5
All*	Total formula weight	31621.0

*Water molecules are not included.