9I3T

Dark-state structure of human medium-wave-sensitive cone opsin (OPN1MW)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Medium-wave-sensitive opsin 1,Soluble cytochrome b562	polymer	492	54622.6	1	UniProt (P04001) UniProt (P0ABE7) Pfam (PF00001)	Homo sapiens (human)	Green cone photoreceptor pigment,Green-sensitive opsin,GOP,Cytochrome b-562
2	B (H)	BAG2 Anti-BRIL Fab Heavy Chain	polymer	231	24539.3	1		synthetic construct
3	C (L)	BAG2 Anti-BRIL Fab Light Chain	polymer	215	23541.2	1		synthetic construct
4	D (A)	RETINAL	non-polymer		284.4	1	Chemie (RET) PubChem (638015) PubChem (5381765)

Sequence modifications

A: 1 - 899 (UniProt: P04001)
A: 900 - 1005 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 906	Met 29	engineered mutation
Ile 1001	His 124	engineered mutation
Leu 1005	Arg 128	engineered mutation

A: 254 - 364 (UniProt: P04001)

PDB	External Database	Details
Leu 365	-	expression tag
Glu 366	-	expression tag
Val 367	-	expression tag
Leu 368	-	expression tag
Phe 369	-	expression tag
Gln 370	-	expression tag
Gly 371	-	expression tag
Pro 372	-	expression tag
Asp 373	-	expression tag
Tyr 374	-	expression tag
Lys 375	-	expression tag
Asp 376	-	expression tag
Asp 377	-	expression tag
Asp 378	-	expression tag
Asp 379	-	expression tag
Lys 380	-	expression tag
Gly 381	-	expression tag
Ala 382	-	expression tag
Gly 383	-	expression tag
Ala 384	-	expression tag
Thr 385	-	expression tag
Glu 386	-	expression tag
Thr 387	-	expression tag
Ser 388	-	expression tag
Gln 389	-	expression tag
Val 390	-	expression tag
Ala 391	-	expression tag
Pro 392	-	expression tag
Ala 393	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	102703.1
Non-Polymers*	Number of molecules	1
	Total formula weight	284.4
All*	Total formula weight	102987.6

*Water molecules are not included.