9I3P
CryoEM structure of the Themis:Grb2 complex with bound ProMacrobody 256
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Growth factor receptor-bound protein 2 | polymer | 224 | 26198.4 | 1 | UniProt (P62993) Pfam (PF00018) Pfam (PF00017) | Homo sapiens (human) | Adapter protein GRB2,Protein Ash,SH2/SH3 adapter GRB2 |
| 2 | B (B) | Protein THEMIS | polymer | 563 | 64723.3 | 1 | UniProt (Q8N1K5) Pfam (PF12736) | Homo sapiens (human) | Thymocyte-expressed molecule involved in selection |
| 3 | C (C) | ProMacrobody 256 | polymer | 478 | 52516.0 | 1 | UniProt (P0AEY0) Pfam (PF01547) | synthetic construct | MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP |
| 4 | D (E) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) |
Sequence modifications
A: 1 - 217 (UniProt: P62993)
C: 1 - 116 (PDB: 9I3P)
C: 119 - 478 (UniProt: P0AEY0)
| PDB | External Database | Details |
|---|---|---|
| Lys 218 | - | expression tag |
| His 219 | - | expression tag |
| His 220 | - | expression tag |
| His 221 | - | expression tag |
| His 222 | - | expression tag |
| His 223 | - | expression tag |
| His 224 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Pro 117 | - | linker |
| Pro 118 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 143437.7 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| All* | Total formula weight | 143780.0 |
