9I1J
Cryo-EM structure of mouse RNF213:UBE2L3 transthiolation intermediate, chemically stabilized, and ATPgS
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | E3 ubiquitin-protein ligase RNF213 | polymer | 4836 | 552623.4 | 1 | UniProt (E9Q555) Pfam (PF00097) Pfam (PF20173) | Mus musculus (house mouse) | Mysterin,RING finger protein 213,RING-type E3 ubiquitin transferase RNF213 |
| 2 | B (B) | Ubiquitin-conjugating enzyme E2 L3 | polymer | 166 | 19197.9 | 1 | UniProt (P68036) Pfam (PF00179) | Homo sapiens (human) | E2 ubiquitin-conjugating enzyme L3,L-UBC,UbcH7,Ubiquitin carrier protein L3,Ubiquitin-conjugating enzyme E2-F1,Ubiquitin-protein ligase L3 |
| 3 | C (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) | |||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E, F (A) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 6 | G, H (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) |
Sequence modifications
A: 339 - 5148 (UniProt: E9Q555)
B: 1 - 154 (UniProt: P68036)
| PDB | External Database | Details |
|---|---|---|
| Met 326 | - | initiating methionine |
| Ala 327 | - | expression tag |
| Ser 328 | - | expression tag |
| Trp 329 | - | expression tag |
| Ser 330 | - | expression tag |
| His 331 | - | expression tag |
| Pro 332 | - | expression tag |
| Gln 333 | - | expression tag |
| Phe 334 | - | expression tag |
| Glu 335 | - | expression tag |
| Lys 336 | - | expression tag |
| Gly 337 | - | expression tag |
| Ser 338 | - | expression tag |
| - | Val 4264 | deletion |
| - | Arg 4265 | deletion |
| - | Asn 4266 | deletion |
| - | Arg 4267 | deletion |
| Gly 5149 | - | expression tag |
| Gly 5150 | - | expression tag |
| Gly 5151 | - | expression tag |
| His 5152 | - | expression tag |
| His 5153 | - | expression tag |
| His 5154 | - | expression tag |
| His 5155 | - | expression tag |
| His 5156 | - | expression tag |
| His 5157 | - | expression tag |
| His 5158 | - | expression tag |
| His 5159 | - | expression tag |
| His 5160 | - | expression tag |
| His 5161 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -11 | - | expression tag |
| Trp -10 | - | expression tag |
| Ser -9 | - | expression tag |
| His -8 | - | expression tag |
| Pro -7 | - | expression tag |
| Gln -6 | - | expression tag |
| Phe -5 | - | expression tag |
| Glu -4 | - | expression tag |
| Lys -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ser 17 | Cys 17 | conflict |
| Ser 137 | Cys 137 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 571821.3 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1516.7 | |
| All* | Total formula weight | 573338.0 |
