9I1I

Cryo-EM structure of mouse RNF213 (WB3/WB4 + ATP)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	E3 ubiquitin-protein ligase RNF213	polymer	5161	586543.6	1	UniProt (E9Q555) Pfam (PF00097) Pfam (PF20173)	Mus musculus (house mouse)	E3 ubiquitin-lipopolysaccharide ligase RNF213,Mysterin,RING finger protein 213
2	B, F (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
3	C (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	D, E (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 1 - 5148 (UniProt: E9Q555)

PDB	External Database	Details
Gln 2449	Glu 2449	engineered mutation
Gln 2806	Glu 2806	engineered mutation
Gly 5149	-	expression tag
Gly 5150	-	expression tag
Gly 5151	-	expression tag
His 5152	-	expression tag
His 5153	-	expression tag
His 5154	-	expression tag
His 5155	-	expression tag
His 5156	-	expression tag
His 5157	-	expression tag
His 5158	-	expression tag
His 5159	-	expression tag
His 5160	-	expression tag
His 5161	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	586543.6
Non-Polymers*	Number of molecules	5
	Total formula weight	1169.5
All*	Total formula weight	587713.0

*Water molecules are not included.